miR-1 Inhibits the Ferroptosis of Chondrocyte by Targeting CX43 and Alleviates Osteoarthritis Progression

Figure 4

miR-1 regulated chondrocyte functions by targeting CX43. (a–c) EdU and CCK-8 assays used to detect the proliferation ability of chondrocytes. (d–g) Scratch wound healing and transwell assays used to detect the functional role of CX43 on the migration ability of chondrocytes. (h–i) Annexin V/FITC/PI double-staining assay used to verify the role of CX43 in chondrocyte apoptosis. (j) The protein expression levels of aggrecan, Col2, Sox9, MMP13, BCL-2, and cleaved caspase-3 in chondrocytes after CX43 transfection. Scale bar = 200 µm. Data were presented as the mean ± SD of at least three replicates. , and .

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