miR-1 Inhibits the Ferroptosis of Chondrocyte by Targeting CX43 and Alleviates Osteoarthritis Progression

Figure 5

miR-1 inhibited ferroptosis in chondrocytes by targeting CX43, attenuating matrix degradation and inflammation. (a) Western blot assay used to detect the protein expression levels of CX43, aggrecan, Col2, Sox9, MMP13, BCL-2, and cleaved caspase-3 in OA and normal cartilage samples. (b) The expression levels of CX43 and antiferroptosis proteins (GPX4, SLC7A11) in chondrocytes transfected with or without Agomir-1 and CX43. (c) C11 BODIPY fluorescent probe used to detect the level of intracellular lipid-ROS in chondrocytes. Red indicated unoxidized dye, green indicated oxidized dye. (d) Intracellular ROS in chondrocytes detected by DCFHDA. (e–f) Fe²⁺ and MDA concentrations in chondrocytes were measured using the iron assay kit and MDA assay kit. Scale bar = 200 µm. Data were presented as the mean ± SD of at least three replicates. and .

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