Age-Related Changes in Female Murine Reproductive Mucosa with respect to γδ T Cell Presence

Figure 1

The analysis of vaginal γδ T cell numbers in young and old mice. (a) Microscopic visualization of γδ T cells in the vaginas of Tcrd-H2BeGFP mice. Side projections of vaginal wall with GFP-expressing γδ T cells. GFP shown in green, laminin 5 in red, and stromal collagen in blue. Scale  μm. (b, c) Quantification of γδ T cells in murine vagina in young and old mice using microscopy (b) and cytometry (c). Each spot represents an individual young (black, 2 months old) and old mouse (orange, 18 months old). mice per group (microscopy); mice per group (cytometry). Bars and numbers show medians. No statistically significant differences between groups (Mann–Whitney test).

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