Sex-Specific Immune Responses to Seasonal Influenza Vaccination in Diabetic Individuals: Implications for Vaccine Efficacy

Figure 2

The evaluation of subsets of conventional dendritic cells (cDC). (a) Live single splenic cell suspension was gated for B220/CD45R negative population on which the MHCII+CD11c+ subset had been gated out. On that, CD8a+CD11b− and CD8a−CD11b+ cDCs were gated out to get B220−MHCII+CD11c+CD11b−CD8a+ (cDC1) and B220−MHCII+CD11c+CD11b+CD8a− (cDC2) population percentage. CD80+CD86+ positive cDC1 and cDC2 population percentages have also been analyzed. (b) The population percentage of cDC1 and (c) cDC2. The percentage exhibited in the Y axis is the total percentage within the 10,000 splenocytes analyzed. Hence, for absolute number conversion of this cDC percentage: 1% on Y axis = 100 cDC. (d) The fold change of population percentage of cDC1 and cDC2. (e) The level of CD80/86 double-positive cDC1 and cDC2 population percentages have been determined. (f) Mean fluorescence intensity (MFI) of MHCI and (g) DEC205 on cDC1 and cDC2 populations analyzed for determining the expression level. D−, nondiabetic groups; D+, diabetic groups. Data in graphs are the representative images derived from at least four independent experiments (, , and ). For each group n = 5, the error bar indicates the standard deviation.

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