Exosome Derived from Human Umbilical Cord Mesenchymal Cell Exerts Immunomodulatory Effects on B Cells from SLE Patients

Figure 5

MiR-155 modulates SHIP-1 expression and the downstream ERK kinase pathway after BCR and FcγRIIB coligation. (a) Western blot analysis was used to detect the protein level of SHIP-1 after coculture with hucMscs and exosomes. β-tubulin was used as an internal control. (b) Western blot analysis was used to detect the protein level of SHIP1 after transfection with agomir-155 or agomir-NC after intervention with hucMSCs-Exo. β-Tubulin was used as an internal control. After incubation with hucMSCs-Exo and transfection with agomir-155 or agomir-NC, whole-cell lysates were probed with antiphosphorylated ERK, stripped, and reprobed with anti-total ERK. β-Tubulin was used as an internal control. Ratio: p-ERK/total ERK determined by Bio-Rad Quantity One software.

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