Research Article
Exosome Derived from Human Umbilical Cord Mesenchymal Cell Exerts Immunomodulatory Effects on B Cells from SLE Patients
Figure 6
Inhibiting B cell activation and inflammation by upregulating the target genes of miR-155. (a) B cell apoptosis was detected by Annexin V-FITC after transfection of antagomir-155 and antagomir-NC. (b) SCFE was used to detect the proliferation of B cells after transfection of antagomir-155 and antagomir-NC. (c) antagomir-155 and hucMSCs-Exo were cocultured with PBMC cells, and B cells were sorted by CD19. Then the activation of 0–36 hr B cells was detected by flow cytometry using CD69. (d) antagomir-155 and hucMSCs-Exo were cocultured with PBMC for a certain time. After centrifugation, the supernatant was collected, and the cytokine levels were detected by multipellet flow immunofluorescence luminescence assay. Exosomes were isolated from hucMSC. Data are expressed as the mean ± SEM. , , .
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