SEMA5A-PLXNB3 Axis Promotes PDAC Liver Metastasis Outgrowth through Enhancing the Warburg Effect

Figure 1

SEMA5A is secreted from M2 macrophages in PDAC liver metastatic niches. (a) Abundance and distribution of infiltrated TAMs in liver metastasis of PDAC were measured by IHC-P staining on M1 macrophage markers (CD163 and IBA1) or M2 macrophage markers (MHCII and CD11c) ( patients, 3 fields assessed per sample). Scale bars, 100 μm. (b) The SUV difference between patients bearing TAMs slightly infiltrated metastatic niches () and TAMs severely infiltrated ones (). ; two tailed unpaired -test, . (c) The Kaplan-Meier analysis on patients bearing TAMs slightly infiltrated metastatic niches () and TAMs severely infiltrated ones () was performed. (d) Real-time PCR measured expressions of secreted semaphorin family in TAMs slightly or severely infiltrated metastatic niches ( samples per group). (e) IHC-P staining on SEMA5A demonstrating its distribution in liver metastatic niches of PDAC ( patients, 3 fields assessed per sample). Scale bars, 100 μm. (f, g) The relationship of CD163 expression and SEMA5A (f) or SEMA5B (g) was measured ( samples). (h) IF staining on SEMA5A and CD163 in PDAC liver metastatic niches displaying their colocation situations ( patients, 3 fields assessed per sample). SEMA5A, green; CD163, red; DAPI, blue; scale bars, 100 μm.

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