SEMA5A-PLXNB3 Axis Promotes PDAC Liver Metastasis Outgrowth through Enhancing the Warburg Effect

Figure 4

SEMA5A-PLXNB3 axis promotes liver metastasis in vivo. (a, b) Relative cell viability of PANC1^shCTRL or PANC1^shPLXNB3 cells cultured with or without M1 TAM (a) or M2 TAM (b) conditional medium measured by CCK-8 kit ( repeats per group, , repeat measures ANOVA). ; NS: no significance. (c, d) Relative cell viability of BxPC3^shCTRL or BxPC3^shPLXNB3 cells cultured with or without M1 TAM (c) or M2 TAM (d) conditional medium measured by CCK-8 kit ( repeats per group, , repeat measures ANOVA). ; NS: no significance. (e) Relative cell viability of PANC1 was cultured in M2 TAM conditional medium experienced IP of SEMA5A. ( repeats per group, , repeat measures ANOVA). . (f) Structure of SMEA5A full length and SEMA5A-ΔTSP. (g) Relative cell viability of AsPC1^PLXNB3-OE in the presence or absence of SEMA5A and SEMA5A-ΔTSP ( repeats per group, , repeat measures ANOVA). . (h) EdU staining was performed on AsPC1^PLXNB3-OE in the presence or absence of SEMA5A and SEMA5A-ΔTSP (3 fields assessed per sample). EdU, red; DAPI, blue; scale bars, 50 μm. (i, j) Cell survival ability was assessed on PANC1 (i) and BxPC3 (j) cell line with administration of rSEMA5A or SEMA5A-ΔTSP ( repeats per group, , repeat measures ANOVA). ; .

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