Bone Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorated Lipopolysaccharide-Induced Lung Injury Via the miR-21-5p/PCSK6 Pathway

Figure 1

Effects of LPS stimulation on the mRNA and protein levels in human BEAS-2B cells, and the identification of PCSK6 shRNAs and overexpression vector. (a) RT-qPCR was used to examine the relative mRNA levels of PCSK6 at 0, 12, 24, and 48 hr in human BEAS-2B cells treated with LPS. (b, c) Western blotting was used to examine the protein levels of PCSK6 in human BEAS-2B cells treated with LPS at 0, 12, 24, and 48 hr. (d–f) PCSK6 shRNAs significantly suppressed the mRNA and protein levels of PCSK6 in human BEAS-2B cells. (g–i) Both mRNA and protein levels of PCSK6 were overexpressed in oePCSK6-transfected cells. , , and vs. 0 hr/shNC/oeNC.

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