Bone Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorated Lipopolysaccharide-Induced Lung Injury Via the miR-21-5p/PCSK6 Pathway

Figure 3

Identification of BMSC-sEV and sEV uptake in human BEAS-2B cells. (a) Identification of BMSCs using flow cytometry. The following antibodies were used to determine the BMSCs phenotype: CD34 FITC, CD45 FITC, CD90 FITC, and CD105 FITC. (b) Nanoparticle tracking analysis (NTA) results of enriched BMSC-sEVs. (c) TEM was used to examine the morphology of BMSC-sEVs. (d) Western blotting was performed to determine the protein contents of CD63 and CD81 in BMSC-sEVs. (e) The uptake of BMSC-sEVs labeled with PKH-67 by human BEAS-2B cells was determined using immunofluorescence.

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