Bone Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorated Lipopolysaccharide-Induced Lung Injury Via the miR-21-5p/PCSK6 Pathway

Figure 5

miR-21-5p regulated PCSK6 by binding to the 3′-UTR of PCSK6. (a) RT-qPCR was used to examine the expression of PCSK6 in BEAS-2B treated with miNC, an miR-21-5p inhibitor, and an miR-21-5p mimic for 48 hr. (b) RT-qPCR was used to examine the expression of miR-21-5p in BEAS-2B with the treatment of miNC, miR-21-5p inhibitor, and miR-21-5p mimic for 48 hr. (c) Illustration of the has-miR-21-5p-binding sequence in the 3′-UTR of WT PCSK6 and its change in the mutant PCSK6. (d) The luciferase activity in cells expressing WT and mutant PCSK6 was treated with miNC, an miR-21-5p inhibitor, and an miR-21-5p mimic. vs. miNC.

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