S100A9 Regulated M1/M2 Macrophage Polarization in Interleukin-10-Induced Promotion of Malignant Pleural Effusion

Figure 2

IL-10 regulates M1/M2 macrophage polarization in MPEs. (a) Representative flow cytometric dot plots (left panel) and statistical comparisons (right panel) of M2 macrophages (CD206⁺MHC-II⁻) and M1 macrophages (CD206⁻MHC-II⁺) in MPEs of WT and IL-10^−/− mice (n = 6). (b) Comparisons of mRNA expression of iNOS, IL-12β, Arg-1, and CD206 in WT and IL-10^−/− BMDMs activated under the M1 or M2 condition (each n = 4). (c) Migration capacity of MC38 cells cocultured with WT and IL-10^−/− mouse MPE macrophages was analyzed in vitro using a transwell coculture system; the representative images (left panel) and statistically significant numbers of migrated cells (right panel) are shown (n = 4). (d) MC38 cells were cocultured with macrophages from MPE, and the apoptosis of MC38 cells was evaluated. Representative flow cytometric dot plots (left panel) and comparisons (right panel) of the apoptotic fraction of MC38 cells cocultured with macrophages from MPEs of WT and IL-10^−/− mice (n = 4). Data are presented as mean ± SD. , , , compared by Student’s t test.

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