S100A9 Regulated M1/M2 Macrophage Polarization in Interleukin-10-Induced Promotion of Malignant Pleural Effusion

Figure 3

Downregulation of S100A9 expression is associated with M1/M2 macrophage polarization in vitro. (a) Heatmap shows a differential expression of mRNAs in MPE macrophages between WT and IL-10^−/− mice. The red color indicates a relatively high expression, and the green color indicates a relatively low expression. (b) Comparison of mRNA expression of S100A9, Spp1, Ppbp, Hspa1a, G0s2, and Cxcr4 in MPE macrophages from WT and IL-10^−/− mice (verified by quantitative RT–PCR, each n = 5). (c) Expression of S100A9 was analyzed by Western blot in MPE macrophages from WT and IL-10^−/− mice. Band densities of samples were analyzed and expressed as a fold change of normalized results from corresponding control cells (n = 5). (d) Comparison of mRNA expression of iNOS, IL-12β, Arg-1, and CD206 in WT BMDMs activated under the M1 or M2 condition in the presence of siCtr or siS100A9. Data are presented as mean ± SD. , , , compared by Student’s t test.

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