Necrostatin-1 Alleviates Diffuse Pulmonary Haemorrhage by Preventing the Release of NETs via Inhibiting NE/GSDMD Activation in Murine Lupus

Figure 3

Nec-1 alleviated lupus pulmonary haemorrhage. (a) The modelling process of C57BL/6 mice. Model and Nec-1 group mice received the same dose of pristane/phosphate-buffered saline (PBS) via intraperitoneal injection (i.p.) on day 0, followed by daily i.p. injection of PBS/Nec-1 until sacrifice on day 14. (b) The weight-change trends of mice in different groups. (c) Survival curves of mice in different groups. (d) Morphology of lung tissues in different groups. (e) Representative images of the haematoxylin and eosin-stained lung tissues of mice from different groups. (f) Pathological staining score of mice lung tissue from different groups. (g, h) Levels of IL-1β and IL-6 in the bronchoalveolar lavage from different groups. (i) Wet/dry weight ratio of the mice lung tissue of different groups was calculated using ImageJ. Data are presented as deviation. Values are expressed as error of the mean of three independent experiments (, , , and ).

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