Necrostatin-1 Alleviates Diffuse Pulmonary Haemorrhage by Preventing the Release of NETs via Inhibiting NE/GSDMD Activation in Murine Lupus

Figure 4

Nec-1 prevented the release of neutrophil extracellular traps (NETs) in mice with diffuse alveolar haemorrhage. (a) Representative images of the immunohistochemistry of MPO from different groups. (b) The positive rate of MPO expression in different groups was calculated using ImageJ. (c) The mean fluorescence intensity of SYTOX Green-stained extracellular DNA in the bronchoalveolar lavage (BAL) of mice was measured using a microplate reader. (d) The mean fluorescence intensity of SYTOX Green-stained extracellular DNA in the peripheral blood supernatant was measured using a microplate reader. (e) Representative immunofluorescence images of the nucleus and myeloperoxidase (MPO) in lung tissues from different groups. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) (blue). MPO was stained with a fluorescent secondary antibody (red). The merge channel was mixed with two different colours. (f) The average fluorescence intensity of MPO in different groups was calculated using ImageJ. Data are presented as deviation. Values were expressed as error of the mean of three independent experiments (, , and ).

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