Necrostatin-1 Alleviates Diffuse Pulmonary Haemorrhage by Preventing the Release of NETs via Inhibiting NE/GSDMD Activation in Murine Lupus

Figure 5

Nec-1 prevents the release of neutrophil extracellular traps (NETs) by inhibiting neutrophil elastase (NE). (a) Representative images of the immunohistochemistry of NE in different groups. The sepia areas indicate active NE cells. (b) NE-positive rate of immunohistochemistry in different groups was calculated using ImageJ. (c) Representative immunofluorescence images of the nucleus, NE, and MPO in the lung tissues of different groups. Nuclei were stained by DAPI (blue), whereas MPO and NE were stained with antibody immunofluorescence separately. (d) The average fluorescence intensity of NE in different groups was calculated using ImageJ. (e) Representative western blot images of Gasdermin D (GSDMD), N-Gasdermin D (N-GSDMD), NE, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in different groups. (f) The average grey value of protein NE in different groups was calculated using ImageJ. (g) The average grey value of protein N-GSDMD in different groups was calculated using ImageJ. Data are presented as deviation. Values were expressed as error of the mean of three independent experiments (, , , and ).

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