sNASP Mutation Aggravates to the TLR4-Mediated Inflammation in SLE by TAK1 Pathway

Figure 2

sNASP mutation augments LPS-induced the cytokine IL-6 and TNF-α expression in B6.WT mice. The peritoneal macrophages were treated with LPS for 0.5, 1, and 2 hr that IL-6 and TNF-α levels were analyzed by qPCR ((a)–(c), (g)–(i)) and ELISA ((d)–(f), (j)–(l)), compared between B6.WT and B6.∆sNASP mice. Following stimulation with ((a), (d), (g), (j)) LPS 1 ng/ml, ((b), (e), (h), (k)) LPS 10 ng/ml, and ((c), (f), (i), (l)) LPS 100 ng/ml. Data were shown as means ± SEM (n = 3) of one representative experiment. , .

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