sNASP Mutation Aggravates to the TLR4-Mediated Inflammation in SLE by TAK1 Pathway

Figure 6

sNASP mutation has no influence on TLR4-induced IRF3 activation of the pathway. The expression of phosphorylated and total proteins were measured by western blotting in peritoneal macrophages and compared between ((a)–(c)) B6.lpr and B6.∆sNASP.lpr mice, ((d)–(f)) B6.WT and B6.∆sNASP mice, and mouse β-actin was used as an internal control. Following 0/0.5/1/2 hr stimulation with ((a) and (d)) LPS (1 ng/ml), ((b) and (e)) LPS (10 ng/ml), ((c) and (f)) LPS (100 ng/ml). Data were shown as means ± SEM. , ; independent sample t-test. Results shown are representative of 2–3 independent experiments.

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