Effect of Differently Polarized Human Macrophages on the SH-SY5Y Cells Damaged by Ischemia and Hypoxia In Vitro

Table 1

Effect of macrophage-conditioned media on the relative number of differentiated cells and the average neurite length in SH-SY5Y cultures under serum deprivation conditions.

Day	Parameter	SD	RA	+CM-M1	+CM-M2a	+CM-M2(LS)
3	Differentiated cells (%)	3.2 (0; 6.7)	35.2^† (32.3; 37.8)	6.9^‡ (5.4; 8.3)	6.0^‡ (5.2; 7.9)	14.9^†‡§ (10.0; 19.8)
	Average neurite length (μm)	25.1 (19.3; 34.3)	46.5^† (36.2; 60.7)	34.2^†‡ (27.0; 46.6)	35.3^†‡ (26.9; 46.9)	42.5^†‡§ (32.4; 56.9)
7	Differentiated cells (%)	4.3 (1.5; 6.2)	38.2^† (35.7; 40.6)	5.0^‡ (3.5; 5.6)	5.7^‡ (4.6; 6.4)	12.9^†‡§ (9.6; 15.4)
	Average neurite length (μm)	27.5 (21.3; 38.0)	59.6^† (37.6; 75.6)	31.0^‡ (24.2; 46.4)	34.5^‡ (26.1; 44.0)	37.0^†‡ (29.5; 48.7)

Notes. ^† compared to serum deprivation conditions (1% FBS); ^‡ compared to RA (positive control of differentiation); ^§ compared to CM-M1 and CM-M2a (the Mann–Whitney U test). CM, conditioned medium; RA, retinoic acid; SD, serum deprivation; FBS, fetal bovine serum. Data are presented as median (Q1; Q3 values). The results of three independent experiments are presented.