Effect of Differently Polarized Human Macrophages on the SH-SY5Y Cells Damaged by Ischemia and Hypoxia In Vitro

Table 2

Effect of macrophage CM on the relative number of differentiated cells and the average neurite length in SH-SY5Y cultures under serum deprivation and hypoxic conditions.

Day	Parameter	SD/HC	RA	+CM-M1	+CM-M2a	+CM-M2(LS)
3	Differentiated cells (%)	1.8 (0.0; 2.9)	31.6^† (28.7; 33.4)	6.8^†‡ (6.2; 10.0)	8.3^†‡ (4.6; 11.9)	17.7^†‡§ (15.3; 20.0)
	Average neurite length (μm)	25.2 (20.1; 35.5)	48.7^† (37.8; 63.0)	40.5^† (32.0; 50.7)	40.7^† (29.9; 52.5)	38.3^† (31.9; 57.1)
7	Differentiated cells (%)	0.0 (0.0; 0.0)	35.8^† (32.4; 35.8)	8.0^†‡ (7.0; 8.8)	5.8^†‡ (5.6; 9.5)	14.1^†‡§ (13.3; 15.1)
	Average neurite length (μm)	24.8 (20.7; 28.5)	59.3^† (44.9; 71.3)	41.5^†‡ (32.4; 56.4)	45.5^† (36.1; 59.9)	55.5^†§ (42.5; 64.8)

Notes. ^† compared to serum deprivation and hypoxic conditions (1% FBS, 100 µM CoCl₂); ^‡ compared to RA (positive control of differentiation); ^§ compared to CM-M1; compared to CM-M2a (the Mann–Whitney U test). CM, conditioned medium; RA, retinoic acid; SD/HC, serum deprivation and hypoxic conditions; FBS, fetal bovine serum. Data are presented as median (Q1; Q3 values). The results of three independent experiments are presented.