Antieosinophil Antibodies Alone or in Combination with Antineutrophil Cytoplasmic Antibodies (ANCA) Detected in Different Autoimmune Conditions

Figure 1

Representative immunofluorescence (IF) images of common antineutrophil cytoplasmatic antibody (ANCA) patterns: (a) a cytoplasmic staining pattern (c-ANCA) obtained with serum from antiproteinase-3-positive vasculitis patient; (b) a perinuclear staining pattern (p-ANCA) obtained with serum from antimyeloperoxidase-positive vasculitis patient; (c) an uncommon IF pattern seen in ANCA IF slides representing antieosinophil antibodies (indicated with arrowheads) and negative neutrophils (indicated with arrows); (d) the cells with the two-lobed nuclear appearance and the red autofluorescence of the cell cytoplasm by IIF microscopy represent eosinophils (indicated with an arrow). The stained cell nearby is a neutrophil.

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