c-Myb Dominates TBK1-Mediated Endotoxin Tolerance in Kupffer Cells by Negatively Regulating DTX4

Figure 1

Effect of TBK1 on endotoxin tolerance in KCs in vitro: (a) KCs were randomly divided into three groups. Western blotting was used to compare the levels of p-TBK1 and TBK1 in KCs; (b) qPCR analysis of IL-6 and TNF-α in KCs; (c) IFN-α and IFN-β in KCs culture supernatant were evaluated by ELISA; (d) Western blotting analysis of p-TBK1, TBK1, p-NF-κB, NF-κB, iNOS, and TNF-α expression. Densitometric analysis of the Western blotting data; (e) laser scanning confocal microscopy (LSCM) (400x) showed a higher TBK1 phosphorylation in the ET group; (f) siRNA inhibits TBK1 expression which successfully knocked down the protein and mRNA expression of TBK1; (g) differences in the protein and TBK1 phosphorylation level were found by Western blotting; one-way ANOVA with Dunnett’s multiple comparisons was used. Data are represented as the mean ± SD of at least three independent experiments; , , , .

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