c-Myb Dominates TBK1-Mediated Endotoxin Tolerance in Kupffer Cells by Negatively Regulating DTX4

Figure 2

Effect of TBK1 on endotoxin tolerance in vivo: (a) ten mice in the control, endotoxin tolerance, nonendotoxin tolerance, DMSO, and TBK1-knockdown endotoxin tolerance were observed to plot survival curves. According to the log-rank test, ; (b) serum AST and ALT levels in each group of mice reflect changes in liver function; (c) the level of IL-6 and IFN-β in serum was evaluated by ELISA; (d) TUNEL staining was used to detect the number of apoptotic cells; (e) H&E (400x) staining of the liver showed LPS-induced liver injury; (f) immunofluorescence staining showed that the phosphorylation level of TBK1 was increased in the ET group; (g) immunohistochemical staining (400x) was used to demonstrate TBK1 phosphorylation in the liver tissues of each group; (h) clodronate liposomes were used to delete Kupffer cells in the liver. The control liposome group was 200 μl control liposome and the neutral liposome group was 200 μl neutral liposome; (i) phosphorylation of TBK1 in liver tissue of ET and CL + ET (Kupffer cell-depleted mice) was detected by immunohistochemical staining (400x). Statistical significance was tested using the two-tailed Student’s t-test; data are presented as the mean ± SD of at least three independent experiments; , , , .

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