c-Myb Dominates TBK1-Mediated Endotoxin Tolerance in Kupffer Cells by Negatively Regulating DTX4

Figure 5

Overexpression of c-Myb enhances endotoxin tolerance: (a) lentiviral particles (LV-c-Myb-RNAi) were used to decrease c-Myb expression in RAW264.7 cells. Western blotting was used. Densitometric analysis of the Western blotting data; (b) phosphorylation of TBK1 was detected by immunohistochemical staining (400x); (c) H&E (400x) staining of liver sections in each group. Arrow indicated the infiltration of inflammatory cells; (d) detection of serum ALT and AST levels in each group. One-way ANOVA with Dunnett’s multiple comparisons was used; (e) lentiviral particles (LV-c-Myb) were administered to increase c-Myb expression. Western blotting was used; (f) Western blotting results show the protein expression of NIK, P-P100, p100, and p52. Densitometric analysis of the Western blotting data. The two-tailed Student’s t-test was used to test for statistical significance; data are represented as the mean ± SD of at least three independent experiments; , , .

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