EGF-Receptor against Amphiregulin (AREG) Influences Costimulatory Molecules on Monocytes and T Cells and Modulates T-Cell Responses

Figure 2

T-cell response upon stimulation with a polyclonal antibody (OKT3). Proliferation of T cells was determined by CFSE-assay. The proliferation rate was analyzed for PBMNC treated with AREG only (a). Proliferation in the presence of Mo was triggered by addition of OKT3 to PBMNC cultured as indicated (b). After removal of Mo (c and d), T-cell proliferation was induced with either soluble (c) or cross-linked OKT3 (; d and e) and CD28 (e), respectively. The percentage of apoptotic T cells was assessed by hypoploid DNA (Nicoletti) assay (f; statistical analysis for all charts: Student’s t-test, , ).

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