Small Extracellular Vesicles Secreted by iPSC-Derived MSCs Ameliorate Pulmonary Inflammation and Lung Injury Induced by Sepsis through Delivery of miR-125b-5p

Figure 2

iMSC-sEV significantly alleviated septic lung injury and reduced pulmonary inflammation in rats. (a, b) Lung histopathology examination and lung injury scores as determined by H&E staining. Scale bar, 100 μm. (c) The wet/dry weight ratio in lung tissue. (d) Survival rate of rats in each group within 7 days. The Kaplan–Meier method combined with the log-rank test was used to compare multiple populations. Compared with the sham group, α; compared with CLP group, β; n = 20 for each group. (e) The changes in sepsis score of each group. (f–h) Levels of IL-1β, TNF-α, and IL-6 in BALF were detected by ELISA. (i) Immunohistochemical for CD68 positive macrophage in the lung tissue. Scale bar, 50 μm. (j) The numbers of CD68 positive loci were quantified with ImageJ software. Data presented as mean ± SD. , compared within two groups. n = 6 per group.

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