Small Extracellular Vesicles Secreted by iPSC-Derived MSCs Ameliorate Pulmonary Inflammation and Lung Injury Induced by Sepsis through Delivery of miR-125b-5p

Figure 5

Anti-inflammatory effects of iMSC-sEV on AMs were mediated by miR-125b-5p in vitro and in vivo. (a, b) ELISA examined the level of inflammatory cytokine expression and NF-κB signaling pathway in LPS-treated NR8383 cells transfected with miR-125b-5p mimics. (c, d) Detection of inflammatory cytokines and NF-κB signaling pathway in LPS-treated NR8383 cells with the iMSC-sEV or iMSC-sEV +miR-125b-5p inhibitor. (e) Changes in histologic (H&E staining) and CD68 positive macrophage infiltration in the lung tissue. (f) Lung injury score of rats in each group. (g) The numbers of CD68 positive loci were quantified with ImageJ software. (h) The ELISA analysis showed the concentration of intrapulmonary inflammatory cytokines (IL-1β, TNF-α) in the BALF. (i) Survival rate of rats in each group within 7 days. The Kaplan–Meier method combined with the log-rank test was used to compare multiple populations. Compared with the CLP + NC mimic group, α; n = 20 for each group. Data presented as mean ± SD. , compared within two groups. n = 6 per group.

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