lncRNA HCG11 Promotes Colorectal Cancer Cell Malignant Behaviors via Sponging miR-26b-5p

Figure 2

HCG11 sequesters miR-26b-5p in CRC cells. (a, b) Subcellular fractionation and FISH assays were carried out to examine the cellular distribution of HCG11. (c) Putative miRNAs could bind with HCG11 which were predicted based on ENCORI. (d) RNA pull-down assay was conducted to confirm the interplay between HCG11 and miRNAs. (e) miR-26b-5p expression in different cell lines was tested via qRT-PCR. (f) The binding sites of HCG11 and miR-26b-5p obtained from ENCORI were presented. (g) The overexpression efficiency of miR-26b-5p mimics was detected by qRT-PCR. (h) Luciferase reporter assay tested the interplay between HCG11 and miR-26b-5p. (i) RIP assay tested enrichment of HCG11 or miR-26b-5p in anti-AGO2 or anti-IgG complexes. and .

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