lncRNA HCG11 Promotes Colorectal Cancer Cell Malignant Behaviors via Sponging miR-26b-5p

Figure 3

ARPP19 is the downstream gene of miR-26b-5p in CRC cells. (a) The microT, miRanda, miRmap, PITA, and TargetScan databases jointly predicted underlying target genes of miR-26b-5p. (b) The mRNAs downregulated upon HCG11 knockdown and miR-26b-5p overexpression were screened as the candidates. (c) Expressions of 5 candidate mRNAs in CRC cell lines relative to NCM-460 cells were measured via qRT-PCR. (d) Western blot analyzed the expression of ARPP19 in different cell lines. (e) The binding sites between miR-26b-5p and ARPP19 were acquired after ENCORI prediction. (f) Luciferase reporter assay was done to verify the interaction between miR-26b-5p and ARPP19. (g) The existence of HCG11, miR-26b-5p, and ARPP19 in anti-AGO2 was detected by RIP assay. (h) ARPP19 expression was assessed via qRT-PCR in cells with transfection of sh-HCG11#1 or sh-HCG11#1+miR-26b-5p inhibitor. .

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