Research Article
lncRNA HCG11 Promotes Colorectal Cancer Cell Malignant Behaviors via Sponging miR-26b-5p
Table 1
Primer sequences used in qRT-PCR and sequences of transfection plasmids.
| | Primer sequences used in qPCR | | HCG11 | F: TGACTATTCACTGCGTGGTGG | | R: AGGTCACTCCCAGAAGACAG | | GAPDH | F: GACAGTCAGCCGCATCTTCT | | R: GCGCCCAATACGACCAAATC | | U6 | F: TCCCTTCGGGGACATCCG | | R: AATTTTGGACCATTTCTCGATTTGT | | miR-455-5p | Reverse transcription stem loop primer: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCcgatgt
miRNA stem loop universal reverse primer: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGC
F: tatgtgcctttggact | | miR-26b-5p | Reverse transcription stem loop primer: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCacctat
miRNA stem loop universal reverse primer: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGC
F: ttcaagtaattcagg | | TOB1 | F: CTAACGGCGATCTCCCAAGG | | R: GTGATCTTGTTCGGGCTGCT | | KMT2C | F: GAAGGCAACTGACCCAGGAA | | R: AGACCTGCAGATGTTGCTCC | | ARPP19 | F: GCGGAGGAGCAGAAGGAAAT | | R: GTTGAGGAAGGTCTTGCGGA | | LARP1 | F: ATTTACGGCGGCTGCATCTA | | R: GCCTCGAACTTGGCTAGCAG | | ETF1 | F: GGAGGAGGAGGCGAGAAGAT | | R: GCAGTTCCAAACTCATCCGC |
| | Sequences of transfection plasmids | | sh-NC | 5-CCGGTCATTCTACCATTTGTCTCTTCTCGAGAAGAGACAAATGGTAGAATGATTTTTG-3 | | sh-HCG11#1 | 5-CACCGTCATTCTACCATTTGTCTCTTCTCGAGAAGAGACAAATGGTAGAATGA-3 | | sh-HCG11#2 | 5-CACCAATGTCTTCTAAATATGACCACTCGAGTGGTCATATTTAGAAGACA-3 | | sh-NC | 5-CCGGTTTATCTTCCATTTCCTTCTGCTCGAGCAGAAGGAAATGGAAGATAAATTTTTG-3 | | sh-ARPP19#1 | 5-CACCGTTTATCTTCCATTTCCTTCTGCTCGAGCAGAAGGAAATGGAAGATAAA-3 | | sh-ARPP19#2 | 5-CACCGTTCTTCATTTTTGCTTTAGCCCTCGAGGGCTAAAGCAAAAATGAAGAA-3 | | NC mimics | uucaauacaggauaguuguag | | miR-26b-5p mimics | uucaaguaauucaggauaggu | | NC inhibitor | accuauccuaacuugaagauu | | miR-26b-5p inhibitor | accuauccugaauuacuugaa |
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