Production of Dermatophagoides farinae Having Low Bacterial Content Using Ampicillin

Figure 3

Induction of airway hyperreactivity with ampicillin treated-Dermatophagoides farinae (D.f. + AMP) and untreated D. farinae (D.f.) in vivo. (a) Airway resistance values. (b) Inflammatory cell infiltration in the lung. (c) Hematoxylin and eosin (H&E), periodic acid-schiff (PAS), and Masson’s trichrome staining of the lung sections. (d) Expression of D. farinae-specific immunoglobulin (Ig)-E, IgG1, and IgG2a in mice. Data are presented as the mean ± standard deviation of at least three independent experiments. PBS, phosphate-buffered saline. , .

(a)

(b)

(c)

(d)