β-Glucan Subverts the Function of Myeloid Cells in Neonates

Figure 1

β-glucan did not affect the numbers of newborn myeloid cells. Neonatal mice at postnatal days 3–4 were injected intraperitoneally (i.p.) with 0.5 mg of β-glucan in 50 μl of PBS, or with PBS as a control. Mice were sacrificed and cells were analyzed at day 3 after β-glucan treatment. (a) Experimental design. (b) The flow cytometric gating strategies for MDSCs were displayed. (c) Representative flow cytometric plots (left), and frequencies and numbers (right), of PMN-MDSCs and M-MDSCs in the spleen of neonatal mice after β-glucan treatment (n = 6). (d) Representative flow cytometric plots (left), and frequencies and numbers (right), of PMN-MDSCs and M-MDSCs in the bone marrow of neonatal mice after β-glucan treatment (n = 6). Data represented mean ± SEM from two independent experiments. ns, not significant.

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