β-Glucan Subverts the Function of Myeloid Cells in Neonates

Figure 2

β-glucan compromised the immunosuppressive activity of MDSCs in neonates. (a) Experimental design of MDSC and T cell co-culture experiment. (b) The flow cytometric gating strategies for T cell proliferation. (c) CD3/CD28-inducible proliferation of CD4⁺ and CD8⁺ T cells in the presence of MDSCs. Non-stimulated T cells (no stim) and stimulated T cells without MDSCs were used as controls. T cell proliferation was evaluated using CFSE staining. Representative flow cytometry data (left) and statistical results (right) were shown (n = 4). Data represented mean ± SEM. and .

(a)

(b)

(c)