Research Article
CD4+T and CD8+T Cells in Uterus Exhibit Both Selective Dysfunction and Residency Signatures
Figure 2
Expression of memory and residence markers on CD4+T cells and CD8+T cells from uterus and blood. Cells from uterus and blood were isolated and stained with anti-CD44, CD69, CD103, CD25, and CD27 mAbs. Gated on CD3+CD4+T (a, b) and CD3+CD8+T cells (c, d), and then compare the expression of CD44, CD69, CD103, CD25, and CD27 in uterus and blood. Representative dot plots and pie chart (n = 6) showed that gated on uterine CD3+CD4+CD44+ memory T cells (e) and CD3+CD8+T CD44+ memory T cells (f), and then detected the proportion of non-TRM (CD69−CD103−) and TRM (CD69+CD103+, CD69+CD103−, and CD69−CD103+) cells. Data were shown as mean ± SEM and compared with the Student’s t-test. NS, no significance; ; ; ; .
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