Bystander Effects and Profibrotic Interactions in Hepatic Stellate Cells during HIV and HCV Coinfection

Figure 3

Analysis of ROS production among LX-2 after 2 and 72 hr post-cocultivation or conditioned medium exposition by flow cytometry. (a) tROS generation by LX-2 cells after cocultivation with Jurkat (HIV) was increased to the control (LX-2 cocultivated with uninfected Jurkat) for both 2 and 72 hr, ( and , respectively). (b) tROS generation by LX-2 cells after “cm” from Jurkat (HIV) was higher in comparison with control (LX-2 exposure “cm” from uninfected Jurkat) at 2 hr, . At 72 hr, no differences were observed. (c) mROS production by LX-2 cells after cocultivation with Jurkat (HIV) was greater than control (LX-2 cocultivated with uninfected Jurkat) for both 2 and 72 hr, . (d) mROS generation by LX-2 cells after conditioned “cm” from Jurkat (HIV) remained unchanged for the different conditions evaluated for both 2 and 72 hr.(e, f) Programed cell death levels (PCD) (positive staining for annexin V and/or 7-AAD) among LX-2 after 72 hr of cocultivation and “cm” exposition remained unchanged for the different conditions evaluated. (g) mROS production by HIV-infected Jurkat cells after cocultivation with LX-2 was greater than control (uninfected Jurkat cocultivated with LX-2) for both 2 hr () and 72 hr (). (h) tROS generation by HIV-infected Jurkat cells after cocultivation with LX-2 was increased to the control (uninfected Jurkat cocultivated with LX-2) for 2 hr () but not at 72 hr. cm, conditioned medium; NS, not significant; STS, staurosporine; and TBH, tert-butyl hydroperoxide. Graphics are showing values obtained from three independent experiments. Data are given as the mean ± SD. , , , and .

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