Bystander Effects and Profibrotic Interactions in Hepatic Stellate Cells during HIV and HCV Coinfection

Figure 5

Determination of the role of HCV-infected hepatocytes on LX-2-to-HIV infected Jurkat cells contact. (a) Representative flow cytometry dot plots showing the relative percentages of Huh 7.5 infection after 72 hr. (b) Bar graph representing the percentage of infection obtained (7.2% ± 0.7%). (c) Determination of HCV infection of Huh7.5 cells by production by LX-2 revealed by immunofluorescence with a specific antibody. (d) Relative expression of TGF-β, IL-6, TNF-α, and IL-1β measured by RT-qPCR in Huh7.5 infected cells. (e) Programed cell death levels (PCD) (positive staining for annexin V and/or 7-AAD) among LX-2 cells, and LX-2-Jurkat coculture treated with “cm” from Huh7.5 cells during 72 hr. (f) mROS production by LX-2 cells and LX-2-Jurkat coculture treated with “cm” from Huh7.5 cells during 72 hr. (g) Determination of collagen deposition by LX-2, and LX-2–Jurkat coculture treated with “cm” from Huh7.5 cells during 72 hr, revealed by Sirius Red staining and quantified by OD readings at 550 nm. NS, not significant; STS, staurosporine. Graphics are showing values obtained from three independent experiments. Data are given as the mean ± SD, , , , and .

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