Identification of Specific Cell Surface Markers on Immune Cells of Squirrel Monkeys (Saimiri sciureus)

Figure 7

Blocking of steady state PD-1 and HLA-DR expression on mitogen stimulation of squirrel monkey PBMC (a) and IFN-γ ELISPOT (b): PBMC (1 × 10⁵) were stimulated with Con A (2 µg/mL) for 72 hr in the presence and absence of PD-1 (5 µg/mL) or HLA-DR (Clone L243; 5 µg/mL) blocking antibodies. Stimulation index was calculated by division of absorbance (A540) of PBMC (+Con A) with absorbance of PBMC (−Con A) (a). PBMC (50,000) were stimulated with Con A (2 µg/mL) for 40 hr in the presence and absence of PD-1 (1 µg/mL) or HLA-DR (clone L243; 5 µg/mL) blocking antibodies for IFN-γ ELISPOT (b). In both experiments, an isotype control (IgG1 or IgG2a for PD-1 and HLA-DR, respectively) at the same concentration of PD-1 or HLA-DR was used as a control. Results from three animals are shown. Values are reported as not significant (ns), , or .

(a)

(b)