CT-IGFBP-4 as a Predictive Novel Biomarker of Ischemic Cardiovascular Events and Mortality: A Systematic Review

Table 3

Description of the assay used in CT-IGFBP-4 measurements in the included studies.

S.N.	Author	Assay used for ct-igfbp-4 determination	Detection limit	Sample used	Storage
1.	García-osuna [14]	ELISA (type not specified)	NR	EDTA-obtained plasma	−80°C
2.	Schulz et al. [10]	Sandwich ELISA	0.8 ng/ml	Lithium-heparin obtained plasma	−40°C
3.	Konev et al. [11]	Sandwich ELISA using monoclonal antibodies (IBP163 as capture antibody, and IBP182HRP as detection antibody)	0.15 ng/ml	Lithium-heparin obtained plasma	−80°C
		Cross reactivity: <2%
4.	Postnikov et al. [12]	Sandwich ELISA using monoclonal antibodies (IBP163 as capture antibody, and IBP182 as detection antibody)	0.8 ng/ml	EDTA obtained plasma	−70°C
		Cross-reactivity: <1.5%
		Linearity range: 12–500 ng/mL
5.	Hjortebjerg et al. [13]	Time-resolved-immunoflourimetric assay (TR-IFMA) using monoclonal antibodies IBP163 and IBP182	0.4 ng/ml	EDTA obtained plasma	NR

ELISA, enzyme-linked immuno-sorbent assay. EDTA, ethylene diamine tetra-acetic acid. IBP, insulin binding protein. NR, not reported.