Topography of Lipid Droplet-Associated Proteins: Insights from Freeze-Fracture Replica Immunogold Labeling

Figure 3

FRIL demonstrates the presence of adipophilin and perilipin in lipid droplets. Examples in which adipophilin (a) and perilipin (b) label is seen in the outer phospholipid monolayers (P face) of the lipid droplet. The example in (c) illustrates a convexly and a concavely fractured lipid droplet. Perilipin is localized in the P face of the lipid droplet monolayer, whereas the E face is completely devoid of label. ((a) from a lipid-laden macrophage; (b) and (c) from lipid-laden adipocytes). Bars: 0.2 μm.