Topography of Lipid Droplet-Associated Proteins: Insights from Freeze-Fracture Replica Immunogold Labeling

<table>Adipophilin in cellular membranes. Survey freeze-fracture view of a lipid-laden macrophage immunogold-labeled for adipophilin. Apart from positive labeling in the periphery of lipid droplets prominent label is seen in the P face of the plasma membrane (PL) and ER membranes. The E faces of the ER, mitochondrial and vesicle membranes are devoid of label. Inset: higher magnification of the P face of ER and plasma membrane. M mitochondria. Bar: 0.2 <i>μ</i>m.</table>

Journal of Lipids

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Figure 5

Figure 5: Topography of Lipid Droplet-Associated Proteins: Insights from Freeze-Fracture Replica Immunogold Labeling 