Topography of Lipid Droplet-Associated Proteins: Insights from Freeze-Fracture Replica Immunogold Labeling

Figure 6

Freeze-fractured nuclear membrane in a lipid-laden macrophage after immunogold labeling for adipophilin. Apart from positive labeling of lipid droplets, the P face of the outer nuclear membrane (NOM) is prominently labeled. The E face of the outer nuclear membrane and both fracture faces of the inner nuclear membrane (NIM) are typically devoid of label. Bar: 0.2 μm.