LysoPC and PAF Trigger Arachidonic Acid Release by Divergent Signaling Mechanisms in Monocytes
Figure 1
PAF and [3H]AA release in human monocytes. Prelabeled THP-1 cells were stimulated with PAF, and [3H]AA release was measured by liquid scintillation counting. (a) The time course of PAF- (35 μM) mediated [3H]AA. (b) PAF stimulation for 5 min mediates [3H]AA release in a dose-dependent matter. (c) Shows inhibition of PAF-induced [3H]AA by the sPLA2 inhibitor SB203347 (10 μM) and the cPLA2 inhibitor MAFP (10 μM). The PAF concentration used is 35 μM. Data are expressed as means ± SD of triplicate determinations within separate experiments. Asterisks indicate that values are statistically different from PAF-treated cells (*).