C16-Ceramide Analog Combined with Pc 4 Photodynamic Therapy Evokes Enhanced Total Ceramide Accumulation, Promotion of DEVDase Activation in the Absence of Apoptosis, and Augmented Overall Cell Killing
Figure 2
LCL30 is taken up by SCCVII cells treated with LCL30 or (PDT+LCL30). (PDT+LCL30): following overnight exposure to Pc 4 (250 nM), cells were treated with LCL30 (1 μM) for 2 hours, irradiated with red light (200 mJ/cm2), and incubated for additional 2 hours. For cells treated with LCL30 alone, the incubation time was 4 hours. Following incubations, cells were collected and processed for MS. The LCL30 levels are expressed as actual values (pmol/mg) and are shown as average ± SEM from four independent determinations.
