C16-Ceramide Analog Combined with Pc 4 Photodynamic Therapy Evokes Enhanced Total Ceramide Accumulation, Promotion of DEVDase Activation in the Absence of Apoptosis, and Augmented Overall Cell Killing
Figure 4
PDT, with or without LCL30, has no effect on mitochondrial membrane potential or apoptosis. Following incubations, cells were collected and processed for flow cytometry. JC-1 and Annexin V/PI staining were used to detect mitochondrial membrane potential (a) and apoptosis (b), respectively. See Section 2 for other details. (a) Percentage of cells with depolarized mitochondria is shown in lower right dot plot. (b) Percentage of Annexin V (+)/PI (−) and Annexin V (+)/PI (+) is shown in lower right and upper right dot plot, respectively. The representative data for PDT, with or without LCL30, from two independent experiments are shown.
