Review Article
The Phospholipid Profile of Mycoplasmas
Table 2
The incorporation of radiolabelled fatty acids into the major phospholipids of M. hyorhinis.
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M. hyorhinis cells were grown in the presence of either [3H]-palmitate or [3H]-oleate. Lipids were extracted by the method of Bligh and Dyer [18] and separated by TLC on silica gel plates (Kiesel-gel 60 HR, Merck, Darmstadt, Germany) developed at room temperature by a two-dimensional system described above. The lipid spots were scraped off the plates and analyzed for radioactivity. To determine phosphorus in the phospholipid spots, the method of Zhou and Arthur [20] was used. The results are the average of three independent experiments using different batches of cells. SPM: sphingomyelin; PC: phosphatidylcholine; PG: phosphatidylglycerol; CL: cardiolipin. |