8-Hydroxyeicosapentaenoic Acid Decreases Plasma and Hepatic Triglycerides via Activation of Peroxisome Proliferator-Activated Receptor Alpha in High-Fat Diet-Induced Obese Mice

Figure 2

Hematoxylin and eosin staining of gonadal WAT (a), quantification of adipocyte cross sections (b), and expression of Pparg, Cebpa, and Fabp4 in gonadal WAT (c) in male C57BL/6J-DIO mice fed HFD, HFD with EPA, or HFD with 8-HEPE for 4 wk. Data were collected from H&E-stained sections from five mice; two fields of view were analyzed per mouse, and 10–15 cells per field were analyzed using ImageJ software. Gene expression levels were measured by real-time PCR and normalized against expression of Actb. Values are means ± SDs, . Labeled means without a common letter differ; . HFD: high-fat diet; HEPE: hydroxyeicosapentaenoic acid; EPA: eicosapentaenoic acid.

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