A Transcriptional Regulatory System of the S. cerevisiae OLE1 Gene Responds to Fatty Acid Species and Intracellular Amount, and not Simply Membrane Status

Figure 3

Effect of membrane altering conditions on OLE1 expression in wild type cells. Conditions thought to affect membrane fluidity were examined. Wild type W303-1A cells containing the OLE1-lacZ p62 reporter plasmid were grown overnight in YNBt (1% tergitol) and refreshed into noted condition for four hours. Cells were harvested and β-galactosidase activity determined. At least 5 replicates were performed for each growth condition. (a) Effect of growth on OLE1 expression in minus (“control”) or plus saturated or trans fatty acids (18:2∆9,12 cis is included as positive control). (b) Effect of temperature was examined on OLE1 expression. 30°C is the normal growth temperature of yeast under laboratory conditions, and was used as the “control” condition. Asterisk indicates significant difference ().

(a)

(b)