Targeting the OB-Folds of Replication Protein A with Small Molecules
Figure 7
TDRL-505 inhibits SpPot1(DBD) ssDNA binding. (a) Titration of TDRL-505 in ssDNA-binding reactions with SpPot1(DBD). TDRL-505 was preincubated with SpPot1(DBD) (0, 25, 50, 100 μM) prior to EMSA analysis of binding to the 15-mer oligonucleotide. “Free” ssDNA is indicated with an arrow, and the “Bound” ssDNA is indicated by a bracket. (b) Analysis of TDRL-505 inhibition of SpPot1(DBD). The average of three independent experiments is presented with SD.
