Photoswitching of Site-Selective RNA Scission by Sequential Incorporation of Azobenzene and Acridine Residues in a DNA Oligomer

<table>Structures of the modified DNA bearing more active acridine (<svg height="15.8625" id="M30" style="vertical-align:-3.40982pt" version="1.1" viewbox="0 0 18.975 15.8625" width="18.975" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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l97 -150l56 77q38 52 47 69q11 22 11 37q0 18 -10.5 25t-40.5 10l-21 2v25h250v-25q-55 -4 -91 -31q-33 -25 -97 -115l-80 -111l182 -283q33 -52 53 -70q17 -14 44 -16z" id="x1D417"></path></g>
<g transform="matrix(.012,-0,0,-.012,12.337,15.638)"><path d="M58 524l-21 12q80 152 229 152q73 0 119 -41q45 -41 45 -96q0 -40 -21.5 -74.5t-62.5 -52.5q57 -20 89.5 -68.5t32.5 -109.5q0 -110 -86 -185t-214 -75q-69 0 -110.5 24.5t-41.5 65.5q0 26 18.5 43t46.5 17q45 0 96 -57q39 -43 79 -43q45 0 71.5 34t26.5 88q0 75 -49 121
q-25 23 -56 39q-34 17 -97 38v17q69 21 100 53t31 78q0 103 -99 103q-40 0 -69.5 -19t-56.5 -64z" id="x1D7D1"></path></g>
</svg>) and the two azobenzene residues (<svg height="15.7" id="M31" style="vertical-align:-3.27605pt" version="1.1" viewbox="0 0 21.612499 15.7" width="21.612499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.55)"><path d="M479 676h220v-25q-48 -7 -68 -39l-191 -311v-178q0 -59 16 -76q18 -17 76 -22v-25h-347v25q59 4 76 25q17 20 17 78v136l-180 328q-32 56 -83 59v25h335v-25l-27 -2q-33 -2 -43.5 -7.5t-10.5 -21.5q0 -12 24 -59l120 -232l109 178q41 68 41 102q0 22 -16.5 30t-67.5 12
v25z" id="x1D418"></path></g>
<g transform="matrix(.012,-0,0,-.012,12.337,15.637)"><path d="M14 651v25h312q172 0 267 -88q97 -88 97 -243q0 -77 -30.5 -147t-84.5 -117q-93 -81 -252 -81h-309v25q83 1 83 73v486q0 34 -17.5 48t-65.5 19zM259 598v-498q0 -36 12.5 -50.5t45.5 -14.5q98 0 149 72q23 33 36.5 94t13.5 129q0 138 -41 211q-59 101 -163 101
q-53 0 -53 -44z" id="x1D403"></path></g>
</svg> and <svg height="15.7" id="M32" style="vertical-align:-3.27605pt" version="1.1" viewbox="0 0 20.9625 15.7" width="20.9625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.55)"><path d="M479 676h220v-25q-48 -7 -68 -39l-191 -311v-178q0 -59 16 -76q18 -17 76 -22v-25h-347v25q59 4 76 25q17 20 17 78v136l-180 328q-32 56 -83 59v25h335v-25l-27 -2q-33 -2 -43.5 -7.5t-10.5 -21.5q0 -12 24 -59l120 -232l109 178q41 68 41 102q0 22 -16.5 30t-67.5 12
v25z" id="x1D418"></path></g>
<g transform="matrix(.012,-0,0,-.012,12.337,15.637)"><path d="M609 227h29l-42 -227h-577v25q56 8 70 20q16 13 16 53v478q0 42 -17.5 57t-68.5 18v25h348v-24q-64 -3 -83 -20q-17 -17 -17 -79v-464q0 -33 18.5 -45.5t66.5 -12.5q109 0 170 55q42 39 87 141z" id="x1D40B"></path></g>
</svg>).</table>

Journal of Nucleic Acids

fig4

Figure 4

Figure 4: Photoswitching of Site-Selective RNA Scission by Sequential Incorporation of Azobenzene and Acridine Residues in a DNA Oligomer 