Effect of Steric Constraint at the γ-Backbone Position on the Conformations and Hybridization Properties of PNAs

Table 1

𝑇_𝑚s of PNA-DNA hybrid duplexes containing perfectly matched sequence.

Name	Sequence	𝑇𝑚 (°C)	Δ𝑇𝑚 (°C)
PNA1 (Unmod)	H-GCATGTTTGA-^LLys-NH2	47	—
PNA2 (Ala)	H-GCATGTTTGA-^LLys-NH2	51	+4
PNA3 (Val)	H-GCATGTTTGA-^LLys-NH2	51	+4
PNA4 (Ile)	H-GCATGTTTGA-^LLys-NH2	51	+4
PNA5 (Phe)	H-GCATGTTTGA-^LLys-NH2	51	+4
PNA6 (Phe 3alt)	H-GCATGTTTGA-^LLys-NH2	58	+11
PNA7 (Phe 3con)	H-GCATGTTTGA-^LLys-NH2	60	+13
PNA8 (Val 3alt)	H-GCATGTTTGA-^LLys-NH2	55	+8
PNA9 (Val 3con)	H-GCATGTTTGA-^LLys-NH2	55	+8